Mirror image proteins offer new way to disable Alzheimer's disease

Understanding how proteins interact with their own mirror images enabled a Kobe University research team to design a small mirror protein that disables a causal factor of Alzheimer's disease, amyloid-beta.

Alzheimer's is formed by proteins in brain cells that have lost their natural shape and have become "disordered." The main protein involved in this is called amyloid-beta. It is thought that such disordered proteins attach to other proteins, causing them to become disordered themselves, and together, they form plaques that inhibit the function of brain cells. Kobe University biochemical engineer MARUYAMA Tatsuo explains what makes targeting these proteins so difficult, saying: "There is a gap in how we approach proteins without a fixed structure. Many existing drug design strategies rely on well-defined structures, and we were frustrated by how limited they are when facing more flexible, complex biological targets, such as amyloid-beta."

The key idea in how to approach this challenge came from materials science. Maruyama says, "It occurred to us that we could intercept amyloid-beta proteins by capturing them with small fragments of their mirror images and thus might be able to stop the aggregation of amyloid-beta proteins." Proteins, as well as their individual components called "amino acids," can in theory occur in two forms that are mirror images to each other like the left and right hand, but in nature they are predominantly composed of only one form. It has been known that short chains of "left-handed" and artificially created "right-handed" amino acids can interact and form stable structures, but a rational design exploiting this mechanism has been absent.

In the scientific journal Chemistry - A European Journal, Maruyama and his team now present a systematic study of small model proteins to find out which molecular mechanisms are important for "left-handed" and "right-handed" proteins to bind to each other efficiently. The Kobe University group then used this new-found understanding to design a short "right-handed" amino acid chain that can efficiently bind to the Alzheimer's-causing amyloid-beta protein and under the tested conditions inhibited the protein better than a different currently promising drug candidate molecule. The interaction can be likened to a right and a left hand fitting together, making it impossible for the left arm to grab other things.